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EC-770廠家供應(yīng)2014年升級(jí)傻瓜操作式涂鍍層測(cè)厚儀EC-770宇問傳感

   EC-770涂層測(cè)厚儀,能同時(shí)測(cè)量磁性基材表面(如鋼、鐵等)的非磁性涂鍍層(如油漆、陶瓷、鉻等),以及非磁性金屬基材表面的非導(dǎo)電涂鍍層(如油漆等)。本儀表內(nèi)置高精密雙探頭,利用電磁感應(yīng)和渦流效應(yīng),全自動(dòng)探測(cè)基材屬性,計(jì)算涂鍍層厚度,并通過點(diǎn)陣液晶快速顯示結(jié)果。同時(shí),測(cè)量數(shù)據(jù)可分組保存,并實(shí)時(shí)顯示統(tǒng)計(jì)值。用戶可分別為每組設(shè)置上下限報(bào)警值、零校準(zhǔn)、多點(diǎn)校準(zhǔn)。全新的多點(diǎn)校準(zhǔn)和零校準(zhǔn),讓您非常方便的隨時(shí)進(jìn)行校準(zhǔn)。標(biāo)準(zhǔn)化菜單,確保您非常容易的使用它。特點(diǎn):A.大點(diǎn)陣液晶屏,標(biāo)準(zhǔn)化菜單操作;B.兩種測(cè)量模式:?jiǎn)未危⊿ingle)和連續(xù)(Continuous);C.兩種組模式:直接組(DIR)和通用組(GEN),一個(gè)直接組和四個(gè)通用組。直接組關(guān)機(jī)后數(shù)據(jù)自動(dòng)全部  清除。通用組數(shù)據(jù)將自動(dòng)保存,關(guān)機(jī)不丟失。每組可存儲(chǔ)80個(gè)數(shù)據(jù);D.可零校準(zhǔn)和多點(diǎn)校準(zhǔn)(最多四點(diǎn))。各組有單獨(dú)的零校準(zhǔn)和多點(diǎn)校準(zhǔn),組與組之間不影響;E.用戶可隨時(shí)查看當(dāng)前工作組已測(cè)得的數(shù)據(jù),并刪除指定數(shù)據(jù)或整組數(shù)據(jù);F.實(shí)時(shí)顯示當(dāng)前工作組統(tǒng)計(jì)值:平均值(Mean),最小值(Min),最大值(Max),標(biāo)準(zhǔn)方差(Sdev);G.三種探頭模式: 自動(dòng)(Auto)、磁感應(yīng)(Magnetic)和渦流(Eddy Current);H.可為各組單獨(dú)設(shè)置高低限報(bào)警值,超限時(shí)屏幕指示報(bào)警;I.可開啟或關(guān)閉自動(dòng)關(guān)機(jī)功能J.USB接口可傳輸通用組數(shù)據(jù)到計(jì)算機(jī)K.低電和錯(cuò)誤提示

該公司產(chǎn)品分類: 一氧化碳記錄儀 熱電偶記錄儀 電壓電流記錄儀 溫濕度記錄儀 溫度記錄儀 數(shù)據(jù)記錄儀

CC-118A透明槽加熱型恒溫水浴

    CC-118A透明槽加熱型恒溫水浴可以方便觀察浴槽中的變化。工作溫度為100℃,接泵接口可以用于外循環(huán),溫度穩(wěn)定性符合DIN12876標(biāo)準(zhǔn)。    CC-118A透明槽加熱型恒溫水浴技術(shù)指標(biāo)    內(nèi)槽體積:18L;    加熱功率:2KW;    溫度范圍:室溫~100℃    開口尺寸(mm):302*325    外形尺寸(mm):333*520*335

    訂貨號(hào):2001.0005.04

     

SV壓力傳感器,稱重傳感器(load cell)

我公司(svloadcell@hotmail.com)主要經(jīng)營(yíng)壓力傳感器(load cell)、稱重傳感器,測(cè)力傳感器,拉力傳感器,張力傳感器,壓力變送器(pressure sensor),稱重系統(tǒng)(weighing system),配料稱重系統(tǒng),罐體稱重系統(tǒng),料斗稱重系統(tǒng),電子稱,汽車稱重儀,軸重儀及附件的生產(chǎn)廠家。公司的產(chǎn)品線主要分為:應(yīng)變式測(cè)力/稱重傳感器、應(yīng)變式扭矩/多分力傳感器及壓力變送器、應(yīng)變式流體壓力傳感器及變送器和傳感器用高精度電阻式應(yīng)變計(jì)等產(chǎn)品。其中有各種型式的稱重傳感器,如橋式的,剪切梁式的,單點(diǎn)式,柱式的,輪輻式的,板式的,S型的,平行梁式的,同時(shí)也提供OEM,可能根據(jù)客戶要求自行設(shè)計(jì)各類稱重測(cè)力傳感器.

 

  近年來,又開發(fā)了各種稱重測(cè)試臺(tái)、日用衡器用傳感器及電子衡器、高精度稱重傳感器用應(yīng)變計(jì)等新產(chǎn)品系列。

電  話: 0510-88056151 

傳  真: 0510-88056151 

郵件:svloadcell@hotmail.com

郵  編: 214215

公司網(wǎng)站: http://www.load-cell.com.cn

 

RHC-200ATC

用來測(cè)量人或動(dòng)物的尿蛋白,血清含量, 操作方便,精確度高

Inspector Alert α、β、γ和X射線檢測(cè)儀

 儀器介紹

采用一只蓋革-彌勒計(jì)數(shù)管來測(cè)定α、β、γ和X射線輻射  “安全第一”(Safety First)的校準(zhǔn)功能能夠避免校準(zhǔn)人員的輻射接觸  檢測(cè)儀符合歐洲CE認(rèn)證要求 

主要特點(diǎn)

內(nèi)置鹵素淬滅劑GM探測(cè)器,對(duì)α、β射線源的靈敏度很高 四位液晶顯示,可選擇mR/hr、CPM、mSv/hr、CPS或Total/Timer等單位 總計(jì)數(shù)/定時(shí)器功能對(duì)輕微污染進(jìn)行定時(shí)的精確檢測(cè),定時(shí)時(shí)間可選擇1分鐘--    24小時(shí)

技術(shù)參數(shù) 

測(cè)量范圍:mR/hr(毫倫/小時(shí)):0.001—110.0,CPM(每分鐘計(jì)數(shù)):0—300,000    μSv/hr(微希伏/小時(shí)):0.01—1,100,CPS(每秒鐘計(jì)數(shù)):0—5,000,總計(jì)數(shù):       1—9,999,000 效 率:Sr-90(546kev,2.3MeV βmax)約75%  C-14(156kev βmax)約11%    Bi-210(1.2MeV βmax)約64%  Am-241(5.5MeV α)約36% 靈 敏 度:3500CPM/ mR/hr(對(duì)于Cs-137) 精 度:±15% 溫度范圍:-10---+50 電 源:1節(jié)9V堿性電池,電池壽命 200小時(shí)尺寸重量:150×80×30mm 350克(含電池)

應(yīng)用領(lǐng)域

探測(cè)和測(cè)定表面沾污在操作放射性核素時(shí)監(jiān)測(cè)可能存在的放射性暴露量調(diào)查環(huán)境污染測(cè)定惰性氣體及其它低能放射性核素建筑裝飾材料放射測(cè)定 射線危害:低劑量的放射性射線輻射(天然背景輻射的變化范圍),對(duì)人體無害或風(fēng)險(xiǎn)甚低,但達(dá)到一定劑量則會(huì)對(duì)人體有害,可引起癌癥、白內(nèi)障、不孕癥、突變、萎縮效應(yīng)、壽命減短,甚至死亡

應(yīng)用:

偵測(cè)放射性射線,以采取相應(yīng)防護(hù)措施。海關(guān)和邊境巡邏,政府執(zhí)法部門,檢疫檢驗(yàn),應(yīng)急事故處理,核電廠、銀行、政府、實(shí)驗(yàn)室等部門安全巡查,醫(yī)學(xué)廢料處理,消防隊(duì),采礦業(yè),科學(xué)實(shí)驗(yàn),個(gè)人保護(hù),連續(xù)監(jiān)測(cè)

參考信息(來自中國(guó)輻射防護(hù)研究院)

居民的劑量限值為每年1mSv。即0.114μSv/hr。

放射性職業(yè)人員劑量限值為每年20mSv,但任何一年不能超過50mSv。

 

該公司產(chǎn)品分類: 核素識(shí)別儀 便攜式中子測(cè)量?jī)x 便攜式β/γ/中子譜儀 電離室巡測(cè)儀 表面污染測(cè)量?jī)x 多功能輻射測(cè)量?jī)x 個(gè)人劑量報(bào)警儀 便攜式放射性監(jiān)測(cè)儀

2030供應(yīng)COD 上海COD 廠家COD 博取牌CODCr在線自動(dòng)分析儀

 

CODCr在線自動(dòng)分析儀
上海博取儀器有限公司-專業(yè)生產(chǎn)COD檢測(cè)儀
COD檢測(cè)儀
基本原理
水樣、重鉻酸鉀消解溶液、硫酸銀溶液(硫酸銀作為催化劑加入可以更地氧化直鏈脂肪化合物)、以及濃硫酸的混合液加熱到175℃,重鉻酸離子氧化溶液中的有機(jī)物后顏色會(huì)發(fā)生變化,分析儀檢測(cè)此顏色的變化,并把這種變化換算成COD值輸出出來。消耗的重鉻酸離子量相當(dāng)于可氧化的有機(jī)物量。
水樣中還原性的無機(jī)物,例如亞硝酸鹽、硫化物和亞鐵離子,會(huì)和重鉻酸鉀反應(yīng),影響測(cè)量結(jié)果,它們消耗的重鉻酸鉀的量會(huì)記入測(cè)量結(jié)果中,使測(cè)量結(jié)果偏高。
水樣中氯離子的干擾可以通過加入硫酸汞消除,因氯離子能與汞離子形成非常穩(wěn)定的。
 
COD檢測(cè)儀
儀器特點(diǎn)
獨(dú)特的設(shè)計(jì),使本產(chǎn)品較之同類產(chǎn)品具有更低故障率、更低維護(hù)量、更低的試劑消耗量以及更高的性價(jià)比。
1—選擇閥組件:選擇試劑采樣時(shí)序,通道靈活多樣,功能萬變,具有最小死體積,易維護(hù)高壽命等優(yōu)點(diǎn)。
2—微小計(jì)量組件:通過可視光電系統(tǒng)實(shí)現(xiàn)試劑精確計(jì)量,克服了蠕動(dòng)泵泵管由于磨損引起的定量誤差;同時(shí)實(shí)現(xiàn)了微量試劑的精確定量,每劑量?jī)H為1毫升,大大減少了試劑使用量。
3—進(jìn)樣組件:蠕動(dòng)泵負(fù)壓吸入,在試劑與泵管之間總是存在一個(gè)空氣緩沖區(qū),避免了泵管的腐蝕;同時(shí)使得試劑混合更為簡(jiǎn)潔靈活。
4—密封消解組件:高溫高壓消解體系,加快反應(yīng)進(jìn)程,克服了敞口系統(tǒng)腐蝕性氣體揮發(fā)對(duì)設(shè)備的腐蝕。
5—試劑管:采用進(jìn)口改型聚四氟乙烯透明軟管,管徑大于1.5mm,減少了水樣顆粒堵塞幾率。
COD檢測(cè)儀
技術(shù)參數(shù)
Ø         方法依據(jù):國(guó)家標(biāo)準(zhǔn)GB11914-89《水質(zhì)-化學(xué)耗氧量測(cè)定-重鉻酸鉀》。
Ø         測(cè)量范圍:0-6000 mg/L COD。
Ø         準(zhǔn) 確 度:≥100mg/L時(shí),不超過±10%;<100mg/L時(shí),不超過±8mg/L。
Ø         重 復(fù) 性:≥100mg/L時(shí),不超過±10%;<100mg/L時(shí),不超過±6mg/L。
Ø         測(cè)量周期:最小測(cè)量周期為20分鐘,據(jù)實(shí)際水樣,可在5~120min任意修改消解時(shí)間。
Ø         采樣周期:時(shí)間間隔(20~9999min任意可調(diào))和整點(diǎn)測(cè)量模式。
Ø         校準(zhǔn)周期:1~99天任意間隔任意時(shí)刻可調(diào)。
Ø         維護(hù)周期:一般每月一次,每次約30 min。
Ø         試劑消耗:小于0.35元/樣品。
Ø             出:RS-232,4-20mA(選配)。
Ø         環(huán)境要求:溫度可調(diào)的室內(nèi),建議溫度+5~28℃;濕度≤90%(不結(jié)露)。
Ø             源:AC230±10%V,50±10%Hz,5A。
Ø             寸:高1500×寬550×深450(mm)。
Ø             他:異常報(bào)警和斷電不會(huì)丟失數(shù)據(jù);
觸摸屏顯示及指令輸入;
異常復(fù)位和斷電后來電,儀器自動(dòng)排出儀器內(nèi)殘留反應(yīng)物,自動(dòng)恢復(fù)工作狀態(tài)。
 
 
COD檢測(cè)儀儀器安裝
管路安裝請(qǐng)參考以下示意圖進(jìn)行。安裝時(shí),內(nèi)部調(diào)節(jié)閥和外部調(diào)節(jié)閥先全部打開,用手堵住采樣管,開啟潛水泵,慢慢松開采樣管,逐漸關(guān)閉外部調(diào)節(jié)閥,直到采樣管有較強(qiáng)的水流噴出為止,再逐漸關(guān)閉內(nèi)部調(diào)節(jié)閥,直到采樣管內(nèi)的水呈逐滴下流狀或剛剛沒有流出為止。Φ20溢流管與Φ32出水管一定要分開安裝,否則將會(huì)導(dǎo)致采樣管處水壓過大而使設(shè)備無法正常運(yùn)行。也可以將Φ20溢流管的出口直接接入就近的下水道,或把所有同類設(shè)備的溢流管接入到同一根(直徑≥Φ50)下水順暢的管道上。
 
該公司產(chǎn)品分類: COD分析儀 氟離子檢測(cè)儀 磷酸根/硅酸根分析儀 鈉度計(jì) 酸堿濃度計(jì) 濁度儀 余氯分析儀 電導(dǎo)率儀 PH計(jì) 溶氧儀

BD80/120W廣州博勵(lì)德國(guó)凱馳代理大型全自動(dòng)KARCHER洗地機(jī)

 

產(chǎn)地/品牌︰德國(guó)凱馳Karcher
德國(guó)凱馳(廣州博勵(lì))BD80/120W BP大型手推式自走型自動(dòng)洗地吸干機(jī)
產(chǎn)品介紹:KARCHER德國(guó)凱馳(廣州博勵(lì))BD80/120W憑借各120/污水箱的超大水箱容量、的工作視野范圍、強(qiáng)勁耐用的技術(shù),凱馳該款超高性能洗地吸干機(jī),成為當(dāng)今全球大型手推式地面洗地機(jī)的新寵,是您必須在行走洗地條件下的最理想選擇!
 

技術(shù)參數(shù):德國(guó)凱馳Karcher(廣州博勵(lì))全自動(dòng)洗地機(jī)吸干機(jī)
洗地寬度 毫米
800
吸水寬度 毫米
990
/污水箱 升
120/120
洗地效率 平米/小時(shí)
3200
刷子轉(zhuǎn)速 轉(zhuǎn)/分
200
刷子觸地壓力(可調(diào))
36 克/平方厘米
電機(jī)功率 瓦
1660
重量 公斤
170

 
產(chǎn)品描述與應(yīng)用:KARCHER德國(guó)凱馳(廣州博勵(lì))BD80/120W超高性能洗地吸干機(jī),能夠滿足長(zhǎng)時(shí)間、高負(fù)荷工作要求,其對(duì)向轉(zhuǎn)動(dòng)的盤刷,最大化節(jié)約用水,延長(zhǎng)您的工作時(shí)間。
應(yīng)用領(lǐng)域:可廣泛使用于工廠、倉(cāng)庫(kù)、物流中心等地面的清潔與維護(hù)等
廣州市博勵(lì)建材有限公司是德國(guó)凱馳KARCHER授權(quán)中國(guó)代理與凱馳KARCHER戰(zhàn)略級(jí)銷售中心,廣州博勵(lì)主營(yíng)凱馳KARCHER全線產(chǎn)品經(jīng)銷批發(fā)與零售,凱馳清潔設(shè)備包括: KARCHER高壓清洗機(jī),KARCHER高壓水槍, KARCHER洗地機(jī),KARCHER掃地機(jī),KARCHER清掃車,KARCHER吸塵器,KARCHER吸塵吸水機(jī),KARCHER刷地機(jī)單擦機(jī),KARCHER拋光打蠟晶面機(jī),KARCHER地毯清洗機(jī), KARCHER蒸汽清洗機(jī),KARCHER零部件工業(yè)清洗機(jī),KARCHER工業(yè)防爆吸塵器等德國(guó)凱馳KARCHER清潔設(shè)備全系列產(chǎn)品。
廣州博勵(lì):德國(guó)KARCHER授權(quán)華南凱馳銷售中心及凱馳維修服務(wù)中心!
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TTD-15-03PAC投加泵TTD-12-03電磁計(jì)量泵代理銷售

絮凝劑投加泵TTD-15-03電磁計(jì)量泵代理銷售

PAC投加泵TTD-12-03電磁計(jì)量泵代理銷售

水泥助劑添加泵TTD-10-04電磁泵選型銷售

阻垢劑TTD-06-07投加泵計(jì)量泵

緩蝕劑添加泵TTD-03-07德國(guó)TT計(jì)量泵

氫氧化鈉加藥泵TTD-02-07品牌銷售

鹽酸硝酸計(jì)量泵TTD-01-07經(jīng)銷代理

耐腐蝕定量泵TTD-0.5-07小流量計(jì)量泵

 

德國(guó)TT計(jì)量泵分為四大系列TTD手動(dòng)調(diào)節(jié)流量系列 (藍(lán)色)TTM接受脈沖信號(hào)制動(dòng)調(diào)節(jié)流量系列 (淺綠色)TTA接受0/4—20mA信號(hào)自動(dòng)調(diào)節(jié)流量系列(深灰色)TTG機(jī)械隔膜泵系列

TT計(jì)量泵的優(yōu)點(diǎn):1、電磁鐵散熱:電磁鐵運(yùn)行溫度能達(dá)到100攝氏度以上,高溫下電磁線圈磁力減小、剩磁增加。這些因素都會(huì)產(chǎn)生“泵無力”。特殊的散熱翅片和散熱陶瓷應(yīng)用這一問題。2計(jì)量:?jiǎn)蜗蜷y 、單向性雙球防回流。停機(jī)不回留。3、數(shù)字顯示屏。

4、按鍵調(diào)節(jié)。

5、手動(dòng)調(diào)節(jié)流量、數(shù)字面板顯示防腐塑料外殼、散熱陶瓷技術(shù)專用防腐蝕固定螺釘全PTFE隔膜、全四氟軟質(zhì)閥體IP55防護(hù)等級(jí)多種材質(zhì)的泵頭可選:   PP,PVCPVDF,SS316雙球單向閥、PTFE墊片頻率調(diào)節(jié):0-100%重復(fù)精度1%以下。

絮凝劑投加泵TTD-15-03磁計(jì)量泵代理銷售

TTD計(jì)量泵產(chǎn)品說明介紹參數(shù)

德國(guó)TT電磁隔膜計(jì)量泵產(chǎn)品介紹

計(jì)量泵TTD-03-07型號(hào)國(guó)內(nèi)經(jīng)銷商圖片

德國(guó)TTBEST計(jì)量泵常規(guī)型號(hào):

TTD-0.5-07TTD-01-07,TTD-02-07TTD-03-07,TTD-06-07

TTD-10-04 TTD-12-03,TTD-15-03TTD-20-03,TTD-30-03,TTD-50-02

型號(hào)

流量

L/H

壓力

BAR

沖程頻率(N/M

M

KG

W

 

TTD-0.5-07

0.5

7

120

2

2.5

16

220VAC 50HZ

TTD-01-07

1

7

120

2

2.5

16

220VAC 50HZ

TTD-02-07

2

7

120

2

2.5

16

220VAC 50HZ

TTD-03-07

3

7

120

2

2.5

16

220VAC 50HZ

TTD-06-07

6

7

160

2

2.5

16

220VAC 50HZ

TTD-10-04

10

4

200

2

2.5

16

220VAC 50HZ

TTD-12-03

12

3

200

2

2.5

16

220VAC 50HZ

TTD-15-03

15

3

200

2

2.5

16

220VAC 50HZ

TTD-20-03

20

3

200

2

4.5

60

220VAC 50HZ

TTD-30-03

30

3

200

2

4.5

60

220VAC 50HZ

TTD-50-02

50

2

200

2

4.5

60

220VAC 50HZ

1. 防腐的塑料外殼(ABS+聚碳);2. IP65的防護(hù)等級(jí);3. 耐腐蝕的泵頭:PP,PVC,PTFESS 316;4. 耐腐蝕的隔膜:全PTFE隔膜,特殊的工藝結(jié)構(gòu),不壞損;5. 雙球單向閥:密封效果加倍;6. 直觀醒目的操作界面:LED數(shù)碼管顯示當(dāng)前工作頻率,更精確,重復(fù)精度更高;7. 更寬范圍的電源設(shè)計(jì):180-250VAC 50-60HZ 更適用于中國(guó)的國(guó)情;8. 強(qiáng)大的控制功能9. 可附加液位或遠(yuǎn)程控制功能

該公司產(chǎn)品分類: 塞瓦計(jì)量泵 新道茨NEWDOSE 日本安智邁計(jì)量泵 德普羅名特計(jì)量泵 SDP斯得浦計(jì)量泵 美帕斯菲達(dá)計(jì)量泵 意大利EMEC愛米克計(jì)量泵 TTbest精密計(jì)量泵 意大利道茨計(jì)量泵 意大利SEKO計(jì)量泵 米頓羅計(jì)量泵 力高計(jì)量泵 愛力浦計(jì)量泵 計(jì)量泵

AN218004DWDM光譜分析儀AN218004 [1526.05—1563.86 nm] SC/PC

DWDM光譜分析儀AN218004 [1526.05—1563.86 nm] SC/PC DWDM光譜分析儀特征:·PLC 設(shè)備· DWDM(96通道)測(cè)量解決方案· 同一時(shí)間自動(dòng)測(cè)量 CWDM-96通道 (波長(zhǎng) & 功率) · CWDM 彩色圖文掃描· 保存和調(diào)用300數(shù)據(jù)· 軟件可以自行保存數(shù)據(jù)· 自動(dòng)關(guān)機(jī)DWDM光譜分析儀技術(shù)參數(shù): 

技術(shù)參數(shù)

通道數(shù)量

96

通道間隔

50GHz, 100GHz

通道頻率

196.45~191.7THz

最大輸入功率

500mW; 27dBm

帶寬

1526.05~1563.86 nm

光源接頭

SC/PC Standard

測(cè)量速度

4 Sec. (all 96ch.)

電池

鋰聚合物電池, 1800毫安時(shí),3.7

測(cè)量范圍

+10~-40dBm

電池工作時(shí)長(zhǎng)

充滿電后單次使用620分鐘

測(cè)量精度

± 1.0dB @ -40 dBm

電流消耗(Max)

0.25A

顯示屏分辨率

0.01dB

電力消耗

0.925W

顯示單位

dB, dBm,nm,THz

顯示

3.5” TFT-LCD, 16bit color, 240*320

重量

0.6 kg

尺寸

196*95*40 mm

溫度

(環(huán)境條件)

-20 to +55 °C (操作環(huán)境)

濕度

(最大無冷凝)

95%   (操作環(huán)境)

-35 to +65°C (儲(chǔ)存環(huán)境)

85%  (儲(chǔ)存環(huán)境)

http://www.yach.com/Instrument.html   更多光學(xué)儀器儀表
該公司產(chǎn)品分類: 衛(wèi)星天線 慣性導(dǎo)航系統(tǒng) 天線 微波射頻儀器 通訊儀器儀表 光通訊儀器儀表 光學(xué)儀器儀表 光通訊儀器儀表

CSB-E04486h人活化素A(ACV-A)ELISA Kit

1Human Activin A (ACV-A)ELISA KitCatalog No. CSB-E04486h(96T) This immunoassay kit allows for the in vitro quantitative determination of humanACV-A concentrations in serum, plasma. Expiration date six months from the date of manufacture FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.2PRINCIPLE OF THE ASSAYThe microtiter plate provided in this kit has been pre-coated with anantibody specific to ACV-A. Standards or samples are then addedto the appropriate microtiter plate wells with a HorseradishPeroxidase (HRP)-conjugated antibody preparation specific forACV-A and incubated. Then substrate solution A and B are addedto each well. Only those wells that contain ACV-A, HRP-conjugatedantibody will exhibit a change in color. The enzyme-substratereaction is terminated by the addition of a sulphuric acid solutionand the color change is measured spectrophotometrically at awavelength of 450 nm ± 2 nm. The concentration of ACV-A in thesamples is then determined by comparing the O.D. of the samplesto the standard curve.DETECTION RANGE66.6 pg/ml-2000 pg/ml. The standard curve concentrations used forthe ELISA’s were 2000 pg/ml, 1000 pg/ml, 500 pg/ml, 166.6pg/ml,66.6pg/ml.SPECIFICITYThis assay recognizes human ACV-A. No significantcross-reactivity or interference was observed.3SENSITIVITYThe minimum detectable dose of human ACV-A is typically lessthan 16.7 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) wasdefined as the lowest concentration that could be differentiatedfrom zero.MATERIALS PROVIDEDReagent QuantityAssay plate 1Standard(S1-S5) 5HRP-conjugate 1 x 6 mlWash Buffer1 x 15 ml(20×concentrate)Substrate A 1 x 7 mlSubstrate B 1 x 7 mlStop Solution 1 x 7 mlStandard S1 S2 S3 S4 S5Concentration(pg/ml)66.6 166.6 500 1000 20004STORAGE1. Unopened test kits should be stored at 2-8C upon receipt andthe microtiter plate should be kept in a sealed bag. The test kitmay be used throughout the expiration date of the kit, provided itis stored as prescribed above. Refer to the package label for theexpiration date.2. Opened test plate should be stored at 2-8C in the aluminum foilbag with desiccants to minimize exposure to damp air. The kitswill remain stable until the expiring date shown, provided it isstored as prescribed above.3. A microtiter plate reader with a bandwidth of 10 nm or less andan optical density range of 0-3 OD or greater at 450nmwavelength is acceptable for use in absorbance measurement.REAGENT PREPARATION1. Bring all reagents and plate to room temperature for at least 30minutes before use. Unused wells need store at 2-8°C andavoid sunlight.2. Wash Buffer If crystals have formed in the concentrate, warmto room temperature and mix gently until the crystals havecompletely dissolved. Dilute 15 ml of Wash Buffer Concentrateinto deionized or distilled water to prepare 300 ml of WashBuffer.5OTHER SUPPLIES REQUIRED Microplate reader capable of measuring absorbance at 450 nm,with the correction wavelength set at 540 nm or 570 nm. Pipettes and pipette tips. Deionized or distilled water. Squirt bottle, manifold dispenser, or automated microplatewasher. An incubator which can provide stable incubation conditions upto 37°C±0.5°C.SAMPLE COLLECTION AND STORAGE Serum Use a serum separator tube (SST) and allow samplesto clot for 30 minutes before centrifugation for 15 minutes at1000 g. Remove serum and assay immediately or aliquot andstore samples at -20°C. Centrifuge the sample again afterthawing before the assay. Avoid repeated freeze-thaw cycles. Plasma Collect plasma using citrate, EDTA, or heparin as ananticoagulant. Centrifuge for 15 minutes at 1000 g within 30minutes of collection. Assay immediately or aliquot and storesamples at -20°C. Centrifuge the sample again after thawingbefore the assay. Avoid repeated freeze-thaw cycles.Note: Grossly hemolyzed samples are not suitable for use in this assay.6ASSAY PROCEDUREBring all reagents and samples to room temperature before use. It is recommendedthat all samples, standards, and controls be assayed in duplicate. All the reagentsshould be added directly to the liquid level in the well. The pipette should avoidcontacting the inner wall of the well.1. Standard Reconstitute the Standards with 0.5 ml of ddH2O,respectively. Allow the standard to sit for a minimum of 15minutes with gentle agitation prior to use.2. Set a Blank well without any solution. Add 50μl of Standard orSample per well. Standard need test in duplicate.3. Add 50μl of HRP-conjugate to each well (not to Blank well).Mix well and then incubate for 1 hour at 37°C.4. Complete remove the liquid. Then fill each well with WashBuffer (about 200μl), stay for 10 seconds and Spinning. Repeatthe process for a total of three washes. Complete removal ofliquid at each step is essential to good performance. After thelast wash, remove any remaining Wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean papertowels.5. Add 50μl of Substrate A and 50μl of Substrate B to each well,mix well. Incubate for 15 minutes at 37°C. Keeping the plateaway from drafts and other temperature fluctuations in the dark.76. Add 50μl of Stop Solution to each well when the first four wellscontaining the highest concentration of standards developobvious blue color. If color change does not appear uniform,gently tap the plate to ensure thorough mixing.7. Determine the optical density of each well within 10 minutes,using a microplate reader set to 450 nm.CALCULATION OF RESULTSUsing the professional soft "Curve Exert 1.3" to make a standard curve isrecommended, which can be downloaded from our web.Average the duplicate readings for each standard, control, andsample and subtract the average zero standard optical density.Create a standard curve by reducing the data using computersoftware capable of generating a four parameter logistic (4-PL)curve-fit. As an alternative, construct a standard curve by plottingthe mean absorbance for each standard on the x-axis against theconcentration on the y-axis and draw a best fit curve through thepoints on the graph. The data may be linearized by plotting the logof the ACV-A concentrations versus the log of the O.D. and thebest fit line can be determined by regression analysis. Thisprocedure will produce an adequate but less precise fit of the data.If samples have been diluted, the concentration read from thestandard curve must be multiplied by the dilution factor.8LIMITATIONS OF THE PROCEDURE The kit should not be used beyond the expiration date on the kitlabel. Do not mix or substitute reagents with those from other lots orsources. If samples generate values higher than the highest standard,dilute the samples with the appropriate Diluent and repeat theassay. Any variation in operator, pipetting technique, washingtechnique, incubation time or temperature, and kit age cancause variation in binding. This assay is designed to eliminate interference by solublereceptors, binding proteins, and other factors present inbiological samples. Until all factors have been tested in theImmunoassay, the possibility of interference cannot beexcluded.TECHNICAL HINTS Centrifuge vials before opening to collect contents. When mixing or reconstituting protein solutions, always avoidfoaming.9 To avoid cross-contamination, change pipette tips betweenadditions of each standard level, between sample additions,and between reagent additions. Also, use separate reservoirsfor each reagent. When using an automated plate washer, adding a 30 secondsoak period following the addition of wash buffer, and/orrotating the plate 180 degrees between wash steps mayimprove assay precision. To ensure accurate results, proper adhesion of plate sealersduring incubation steps is necessary. Substrate Solution should remain colorless or light blue untiladded to the plate. Keep Substrate Solution protected from light.Substrate Solution should change from colorless or light blue togradations of blue. Stop Solution should be added to the plate in the same order asthe Substrate Solution. The color developed in the wells willturn from blue to yellow upon addition of the Stop Solution.Wells that are green in color indicate that the Stop Solution hasnot mixed thoroughly with the Substrate Solution.

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